Nutrient agar medium pdf

There have been various formulations developed since circa 1910. Meat extract, yeast extract and peptone provide nitrogen, vitamins, amino. Nutrient agar is a generalpurpose medium for the cultivation of nonfastidious microorganisms. Dissolve the dehydrated medium in the appropriate volume of distilled water i. This is a general growth medium and is primarily used for preserving microbial cultures. Nutrient agar medium nam to obtain the discrete colonies of the bacteria present in the specimen or to get the information about cultural characteristics of bacteria on a solid medium. Save some of your budget money by preparing your own nutrient agar plates. Nutrient agar is a complex medium because it contains ingredients with contain unknown amounts or types of nutrients. Nutrient agar and broth are available commercially in powdered freeflowing, homogeneous form. Nutrient medium is a general basic culture medium for less fastidious microorganisms.

Nutrient agar and nutrient broth from oxoid share almost the same medium composition. The main difference between them is that nutrient agar contains a solidifying agent, agar powder that causes the medium to solidify in room temperature, whereas nutrient broth remains in liquid form. Basic practical microbiology why microbiology matters. Nutrient agar na is universally used as a general purpose medium for the cultivation of broad range of bacteria. Food testing for staphylococcus aureus using bbl chromagar staph aureus. Bacteriological media come an a wide range of types. Principle of nutrient agar medium nam bacteria are routinely cultured in a solid medium i. Nutrient broth, nutrient agar, mcconkey, emb usually, bacteria are grown in complex media. Nutrient agar is a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of nonfastidious. Preparation of nutrient agar medium in lab microbiology.

Pdf formulation of alternative culture media for bacterial and. Nutrient agar is a basic culture medium used for maintaining microorganisms 1, for purity checking prior to biochemical or serological testing. Nutrient agar is used as a general purpose medium for the cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids. Check out the preparation of nutrient broth medium in laboratory. Nutrient agar is used for the cultivation of bacteria and for the enumeration of. Nutrient agar is a general purpose, nutrient medium used for the cultivation of microbes supporting growth of a wide range of nonfastidious organisms. Nutrient agar is popular because it can grow a variety of types of bacteria and fungi, and contains many nutrients needed for the bacterial growth.